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Makoto Fujimura

Faculty
Department of Life Sciences
Research Institute of Industrial Technology
Course of Life Sciences
PositionProfessor
Mail
HomepageURL
Birthday
Last Updated :2019/11/07

Researcher Profile and Settings

Association Memberships

  • PESTICIDE SCIENCE SOCIETY OF JAPAN
  • THE PHYTOPATHOLOGICAL SOCIETY OF JAPAN
  • JAPAN SOCIETY FOR BIOSICENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY
  • THE GENETICS SOCIETY OF JAPAN

Research Activities

Research Areas

  • Plant production and environmental agriculture, Plant protection science

Research Interests

    cAMP, Verticillium

Published Papers

  • Luminex Assays for Detection of Fungicide Resistance Mutations in the Plant Pathogens Botrytis cinerea and Corynespora cassiicola. , Shogo NITANDA, Nazuki FUJIMOTO and Makoto FUJIMURA , 73, (4) 14 - 19, 04 , Invited
  • A novel fungicide aminopyrifen inhibits GWT-1 protein in glycosylphosphatidylinositol-anchor biosynthesis in Neurospora crassa, Masahiro Hatamoto, Ryo Aizawa, Yuta Kobayashi, Makoto Fujimura, Pesticide Biochemistry and Physiology, 02 , Refereed
  • Suppressive effect of incorporation of on-site cultivated Indian mustard (Brassica juncea) on Verticillium wilt of eggplant, Makoto Fujimura, Soil Microorganisms, 71, (2) 44 - 48, 10 , Refereed
  • Acetyltransferase activity in Pseudomonas sp. capable of acetylating the C-4 hydroxyl group of nivalenol-type trichothecenes., Tanaka A, Saikawa S, Suzuki T, Echigo A, Maeda K, Sato M, Fujimura M, Tokai T, Usami R, Yoshida Y, Kimura M, Takahashi-Ando N, The Journal of general and applied microbiology, 10 , Refereed
  • Suppressive Effect of Crop Rotation with Broccoli against Verticillium wilt of Eggplant., Kentaro IKEDA, Akiko FURUSAWA and Makoto FUJIMURA, 70, (8) 511 - 514, 08
  • Phenotypic abnormalities of fr, sp, and och-1 single mutants are suppressed by loss of putative GPI-phospholipase A2 in Neurospora crassa, Masayuki Kamei, Masayuki Kamei, Yuko Tsukagoshi, Shinpei Banno, Akihiko Ichiishi, Fumiyasu Fukumori, Makoto Fujimura, Mycoscience, 06 24
  • Involvement of MAK-1 and MAK-2 MAP kinases in cell wall integrity in Neurospora crassa., Kamei M, Yamashita K, Takahashi M, Fukumori F, Ichiishi A, Fujimura M, Bioscience, biotechnology, and biochemistry, 1 - 10, 06 , Refereed
  • Hydroxylations of trichothecene rings in the biosynthesis of Fusarium trichothecenes: evolution of alternative pathways in the nivalenol chemotype., Maeda K, Tanaka A, Sugiura R, Koshino H, Tokai T, Sato M, Nakajima Y, Tanahashi Y, Kanamaru K, Kobayashi T, Nishiuchi T, Fujimura M, Takahashi-Ando N, Kimura M, Environmental microbiology, 04 , Refereed
  • Characterization and distribution of two subtypes of Verticillium longisporum isolated from cabbage fields in Japan, Shinpei Banno, Shinpei Banno, Kentaro Ikeda, Hidenari Saito, Hiroshi Sakai, Toshihiko Urushibara, Toshimasa Shiraishi, Toshimasa Shiraishi, Makoto Fujimura, Journal of General Plant Pathology, 81, 118 - 126, 01 01
  • Crop rotation with broccoli suppresses Verticillium wilt of eggplant, Kentaro Ikeda, Shinpei Banno, Shinpei Banno, Shinpei Banno, Akiko Furusawa, Satoshi Shibata, Kazuhiro Nakaho, Makoto Fujimura, Makoto Fujimura, Journal of General Plant Pathology, 81, 77 - 82, 01
  • Genetic and phenotypic characterization of the heat shock response in Pseudomonas putida., Ito F, Tamiya T, Ohtsu I, Fujimura M, Fukumori F, MicrobiologyOpen, 10 , Refereed
  • Deletion and expression analysis of beta-(1,3)-glucanosyltransferase genes in Neurospora crassa., Kamei M, Yamashita K, Takahashi M, Fukumori F, Ichiishi A, Fujimura M, Fungal genetics and biology : FG & B, 52, 65 - 72, 03 , Refereed
  • Association of Verticillium dahliae and Verticillium longisporum with Chinese cabbage yellows and their distribution in the main production areas of Japan, Ikeda K, Banno S, Watanabe K, Fujinaga M, Ogiso H, Sakai H, Tanaka H, Miki S, Shibata S, Shiraishi T, Fujimura M, J Gen Plant Pathol, 78, 331 - 337, Refereed
  • A mutation in dnaK causes stabilization of the heat shock sigma factor σ32, accumulation of heat shock proteins and increase in toluene-resistance in Pseudomonas putida., Kobayashi Y, Ohtsu I, Fujimura M, Fukumori F, Environmental microbiology, 13, 2007 - 2017, 08 , Refereed
  • Quantitative nested real-time PCR detection of Verticillium longisporum and V. dahliae in the soil of cabbage fields., Banno S, Saito H, Sakai H, Urushibara T, Ikeda K, Kabe T, Kemmochi I, Fujimura M, J Gen Plant Pathol, 77, 282 - 291, Refereed
  • Stem rot of heading cabbage, a new symptom of cabbage Pythium rot, caused by Pythium ultimum var. ultimum, Jpn. J. Phytopathol, 77, 28 - 32, Refereed
  • Mechanism of action of dicarboximide and phenylpyrrole on the stress-response signal transduction pathway, Makoto Fujimura, Journal of Pesticide Science, 35, 351 - 353, 11 03
  • An AP-1-like transcription factor, NAP-1, regulates expression of the glutathione S-transferase and NADH:flavin oxidoreductase genes in Neurospora crassa., Takahashi M, Yamashita K, Shiozawa A, Ichiishi A, Fukumori F, Fujimura M, Bioscience, biotechnology, and biochemistry, 74, (4) 746 - 752, Refereed
  • Characterization of QoI resistance in Botrytis cinerea and identification of two types of mitochondrial cytochrome b gene, Banno S, Yamashita K, Fukumori F, Okada K, Uekusa H, Takagaki M, Kimura M, Fujimura M, Plant Pathol , 58, 120 - 129, Refereed
  • OxyR is involved in the expression of thioredoxin reductase TrxB in Pseudomonas putida., Hishinuma S, Ohtsu I, Fujimura M, Fukumori F, FEMS microbiology letters, 289, 138 - 145, 12 , Refereed
  • ATF-1 transcription factor regulates the expression of ccg-1 and cat-1 genes in response to fludioxonil under OS-2 MAP kinase in Neurospora crassa., Yamashita K, Shiozawa A, Watanabe S, Fukumori F, Kimura M, Fujimura M, Fungal genetics and biology : FG & B, 45, 1562 - 1569, 12 , Refereed
  • 4-O-acetylation and 3-O-acetylation of trichothecenes by trichothecene 15-O-acetyltransferase encoded by Fusarium Tri3., Tokai T, Takahashi-Ando N, Izawa M, Kamakura T, Yoshida M, Fujimura M, Kimura M, Bioscience, biotechnology, and biochemistry, 72, (9) 2485 - 2489, 09 , Refereed
  • A screening system for inhibitors of trichothecene biosynthesis: hydroxylation of trichodiene as a target., Takahashi-Ando N, Ochiai N, Tokai T, Ohsato S, Nishiuchi T, Yoshida M, Fujimura M, Kimura M, Biotechnology letters, 30, 1055 - 1059, 06 , Refereed
  • Genotyping of benzimidazole-resistant and dicarboximide-resistant mutations in Botrytis cinerea using real-time polymerase chain reaction assays., Banno S, Fukumori F, Ichiishi A, Okada K, Uekusa H, Kimura M, Fujimura M, Phytopathology, 98, 397 - 404, 04 , Refereed
  • Genetic analysis of the Neurospora crassa RAD14 homolog mus-43 and the RAD10 homolog mus-44 reveals that they belong to the mus-38 pathway of two nucleotide excision repair systems., Sato M, Niki T, Tokou T, Suzuki K, Fujimura M, Ichiishi A, Genes & genetic systems, 83, (1) 1 - 11, 02 , Refereed
  • Involvement of the osmosensor histidine kinase and osmotic stress-activated protein kinases in the regulation of secondary metabolism in Fusarium graminearum., Ochiai N, Tokai T, Nishiuchi T, Takahashi-Ando N, Fujimura M, Kimura M, Biochemical and biophysical research communications, 363, (3) 639 - 644, 11 , Refereed
  • OS-2 mitogen activated protein kinase regulates the clock-controlled gene ccg-1 in Neurospora crassa., Watanabe S, Yamashita K, Ochiai N, Fukumori F, Ichiishi A, Kimura M, Fujimura M, Bioscience, biotechnology, and biochemistry, 71, (11) 2856 - 2859, 11 , Refereed
  • Genetically engineered Fusarium as a tool to evaluate the effects of environmental factors on initiation of trichothecene biosynthesis., Ochiai N, Tokai T, Takahashi-Ando N, Fujimura M, Kimura M, FEMS microbiology letters, 275, 53 - 61, 10 , Refereed
  • Molecular and genetic studies of fusarium trichothecene biosynthesis: pathways, genes, and evolution., Kimura M, Tokai T, Takahashi-Ando N, Ohsato S, Fujimura M, Bioscience, biotechnology, and biochemistry, 71, 2105 - 2123, 09 , Refereed
  • Involvement of OS-2 MAP kinase in regulation of the large-subunit catalases CAT-1 and CAT-3 in Neurospora crassa., Yamashita K, Shiozawa A, Banno S, Fukumori F, Ichiishi A, Kimura M, Fujimura M, Genes & genetic systems, 82, (4) 301 - 310, 08 , Refereed
  • Roles of putative His-to-Asp signaling modules HPT-1 and RRG-2, on viability and sensitivity to osmotic and oxidative stresses in Neurospora crassa., Banno S, Noguchi R, Yamashita K, Fukumori F, Kimura M, Yamaguchi I, Fujimura M, Current genetics, 51, 197 - 208, 03 , Refereed
  • Identification of OS-2 MAP kinase-dependent genes induced in response to osmotic stress, antifungal agent fludioxonil, and heat shock in Neurospora crassa., Noguchi R, Banno S, Ichikawa R, Fukumori F, Ichiishi A, Kimura M, Yamaguchi I, Fujimura M, Fungal genetics and biology : FG & B, 44, 208 - 218, 03 , Refereed
  • Fusarium Tri4 encodes a key multifunctional cytochrome P450 monooxygenase for four consecutive oxygenation steps in trichothecene biosynthesis., Tokai T, Koshino H, Takahashi-Ando N, Sato M, Fujimura M, Kimura M, Biochemical and biophysical research communications, 353, 412 - 417, 02 , Refereed
  • OxyR regulated the expression of two major catalases, KatA and KatB, along with peroxiredoxin, AhpC in Pseudomonas putida., Hishinuma S, Yuki M, Fujimura M, Fukumori F, Environmental microbiology, 8, 2115 - 2124, 12 , Refereed
  • Molecular biology and biotechnology for reduction of Fusarium mycotoxin contamination, Kimura M, Takahashi-Ando N, Nishiuchi T, Ohsato S, Tokai T, Ochiai N, Fujimura M, Kudo T, Hamamoto H, Yamaguchi I , Pestic. Biochem. Physiol., 86, 117 - 123, Refereed
  • Screening of putative oxygenase genes in the Fusarium graminearum genome sequence database for their role in trichothecene biosynthesis., Tokai T, Koshino H, Kawasaki T, Igawa T, Suzuki Y, Sato M, Fujimura M, Eizuka T, Watanabe H, Kitahara T, Ohta K, Shibata T, Kudo T, Inoue H, Yamaguchi I, Kimura M, FEMS microbiology letters, 251, 193 - 201, 10 , Refereed
  • An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast., Motoyama T, Ohira T, Kadokura K, Ichiishi A, Fujimura M, Yamaguchi I, Kudo T, Current genetics, 47, 298 - 306, 05 , Refereed
  • A two-component histidine kinase of the rice blast fungus is involved in osmotic stress response and fungicide action., Motoyama T, Kadokura K, Ohira T, Ichiishi A, Fujimura M, Yamaguchi I, Kudo T, Fungal genetics and biology : FG & B, 42, 200 - 212, 03 , Refereed
  • A catalytic subunit of cyclic AMP-dependent protein kinase, PKAC-1, regulates asexual differentiation in Neurospora crassa., Banno S, Ochiai N, Noguchi R, Kimura M, Yamaguchi I, Kanzaki S, Murayama T, Fujimura M, Genes & genetic systems, 80, 25 - 34, 02 , Refereed
  • Concordant evolution of trichothecene 3-O-acetyltransferase and an rDNA species phylogeny of trichothecene-producing and non-producing fusaria and other ascomycetous fungi., Tokai T, Fujimura M, Inoue H, Aoki T, Ohta K, Shibata T, Yamaguchi I, Kimura M, Microbiology (Reading, England), 151, 509 - 519, 02 , Refereed
  • Pyrrolnitrin interferes with osmotic signal transduction in Neurospora crassa, Journal of Pesticide Science, 30, (4) 378 - 383, Refereed
  • Cloning and characterization of genes specifically expressed during infection stages in the rice blast fungus., Banno S, Kimura M, Tokai T, Kasahara S, Higa-Nishiyama A, Takahashi-Ando N, Hamamoto H, Fujimura M, Staskawicz BJ, Yamaguchi I, FEMS microbiology letters, 222, 221 - 227, 05 , Refereed
  • Expression in cereal plants of genes that inactivate Fusarium mycotoxins., Higa A, Kimura M, Mimori K, Ochiai-Fukuda T, Tokai T, Takahashi-Ando N, Nishiuchi T, Igawa T, Fujimura M, Hamamoto H, Usami R, Yamaguchi I, Bioscience, biotechnology, and biochemistry, 67, 914 - 918, 04 , Refereed
  • The trichothecene biosynthesis gene cluster of Fusarium graminearum F15 contains a limited number of essential pathway genes and expressed non-essential genes., Kimura M, Tokai T, O'Donnell K, Ward TJ, Fujimura M, Hamamoto H, Shibata T, Yamaguchi I, FEBS letters, 539, 105 - 110, 03 , Refereed
  • Trichothecene nonproducer Gibberella species have both functional and nonfunctional 3-O-acetyltransferase genes., Kimura M, Tokai T, Matsumoto G, Fujimura M, Hamamoto H, Yoneyama K, Shibata T, Yamaguchi I, Genetics, 163, (2) 677 - 684, 02 , Refereed
  • Putative homologs of SSK22 MAPKK kinase and PBS2 MAPK kinase of Saccharomyces cerevisiae encoded by os-4 and os-5 genes for osmotic sensitivity and fungicide resistance in Neurospora crassa., Fujimura M, Ochiai N, Oshima M, Motoyama T, Ichiishi A, Usami R, Horikoshi K, Yamaguchi I, Bioscience, biotechnology, and biochemistry, 67, (1) 186 - 191, 01 , Refereed
  • Effects of iprodione and fludioxonil on glycerol synthesis and hyphal development in Candida albicans., Ochiai N, Fujimura M, Oshima M, Motoyama T, Ichiishi A, Yamada-Okabe H, Yamaguchi I, Bioscience, biotechnology, and biochemistry, 66, (10) 2209 - 2215, 10 , Refereed
  • A Point Mutation in the Two-Component Histidine Kinase BcOS-1 Gene Confers Dicarboximide Resistance in Field Isolates of Botrytis cinerea., Oshima M, Fujimura M, Banno S, Hashimoto C, Motoyama T, Ichiishi A, Yamaguchi I, Phytopathology, 92, 75 - 80, 01 , Refereed
  • Characterization of mutations in the two-component histidine kinase gene that confer fludioxonil resistance and osmotic sensitivity in the os-1 mutants of Neurospora crassa, Ochiai N, Fujimura M, Motoyama T, Ichiishi A, Usami R, Horikoshi K, Yamaguchi I , Pest Manag Sci, 57, (5) 437 - 442, 05 , Refereed
  • Sensitivity to phenylpyrrole fungicides and abnormal glycerol accumulation in os and cut mutant strains of Neurospora crassa, Fujimura M, Ochiai N, Ichiishi A, Usami R, Horikoshi K, Yamaguchi I, J Pestic Sci , 25, 31 - 36, Refereed
  • Fungicide resistance and osmotic stress sensitivity in os mutants of Neurospora crassa, Fujimura M, Ochiai N, Ichiishi A, Usami R, Horikoshi K, Yamaguchi I , Pestic Biochem Physiol, 65, 125 - 133, Refereed
  • Amino-acid alterations in the beta-tubulin gene of Neurospora crassa that confer resistance to carbendazim and diethofencarb., Fujimura M, Kamakura T, Inoue H, Yamaguchi I , Curr Genet , 25, (5) 418 - 422, 05 , Refereed
  • A single amino-acid substitution in the beta-tubulin gene of Neurospora confers both carbendazim resistance and diethofencarb sensitivity, Fujimura M, Oeda K, Inoue H, Kato T , Curr Genet , 21, (4-5) 399 - 404, 04 , Refereed
  • Sensitivity of Neurospora crassa to benzimidazoles and N-phenylcarbamates: effect of amino acid substitutions at position 198 in beta-tubulin., Fujimura M, Kamakura T, Inoue H, Inoue S, Yamaguchi I, Pestic Biochem Physiol, 44, 165 - 173, Refereed

Misc

  • Society awards 2010 (for prominent achievement): Mechanism of action of dicarboximide and phenylpyrrole on the stress-response signal transduction pathway, Journal of Pesticide Science, 35, (3) 363 - 369,   2010 08
  • An easy method to identify 8-keto-15-hydroxytrichothecenes by thin-layer chromatography, ANDO TAKAHASHI Naoko, TOKAI Takeshi, YOSHIDA Minoru, FUJIMURA Makoto, KIMURA Makoto, Mycotoxins, 58, (2) 115 - 117,   2008 07 31 , 4-(p-nitrobenzyl)pyridine (NBP) is often used for detection of trichothecenes developed on a thin-layer chromatography (TLC) plate, because this chromogenic reagent enables specific detection of the 12,13-epoxide group of trichothecenes. After the heat reaction with NBP and cooling at room temperature for several minutes, trichothecenes on the TLC plate are visualized as blue spots by alkalization with tetraethylenepentamine (TEPA). When the TLC plate was left overnight and then sprayed with TEPA, most trichothecenes yielded similar blue colors. However, dark yellowish green spots were give...
  • Current and Future R&D Activities in Agrochemical Area in Korea and Japan (Fungicide), Journal of Pesticide Science, 32,   2007 10
  • (58) Genetic Variations of Fungicide-resistant Isolates in Botrytis cinerea(Abstracts of the Papers Presented at the Annual Meeting of the Society), Banno S., Suzuki T., Uekusa H., Okada K., Fujimura M., Annals of the Phytopathological Society of Japan, 73,   2007 08 25
  • (143) Application of Real-Time PCR for Quantitative Detection of Verticillium longisporum in Cabbage Fields(Abstracts of the Papers Presented at the Annual Meeting of the Society), Saito H., Banno S., Kabe T., Urushibara T., Fujimura M., Annals of the Phytopathological Society of Japan, 73,   2007 08 25
  • Comparison of nuclear DNA content of some yam species (Dioscorea spp.) by flow cytometry, TANAKA H., KUSHIKAWA S., FUJIMURA M., KIMURA Y., 76,   2007 03 24
  • B301 Detection of Fungicide-resistant Mutations Using Real-time PCR with Fluorescent Probes in Botrytis cinerea and Pyricularia oryzae, Banno Shinpei, Suzuki Tomokazu, Uekusa Hidetoshi, Okada Kiyotsugu, Fujimura Makoto, 32,   2007 03 09
  • (156) Detection and Quantification of Verticillium longisporum in Cabbage Fields Using Real-Time PCR(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo), Saito H., Banno S., Sakai H., Urushibara T., Fujimura M., Annals of the Phytopathological Society of Japan, 72,   2006 11 25
  • (165) Application of Real-time PCR Using Fluorescent Hybridization Probes in Detection of Mutations Resistant to Benzimidazoles and Dicarboximides in Botrytis cinerea(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo), Banno S., Mori S., Saito H., Okada K., Uekusa H., Fujimura M., Annals of the Phytopathological Society of Japan, 72, (4) 245 - 246,   2006 11 25
  • (166) Isolation and Characterization of QoI-resistant Botrytis cinerea Isolated from Osaka and Kanagawa(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo), Fujimura M., Yamashita K., Banno S., Takagaki M., Okada K., Uekusa H., Annals of the Phytopathological Society of Japan, 72,   2006 11 25
  • Cloning and characterization of six highly similar endo-1,3-β-glucanase genes in hexaploid wheat, A. Higa-Nishiyama, S. Ohsato, S. Banno, S. Banno, S. H. Woo, M. Fujimura, I. Yamaguchi, M. Kimura, Plant Physiology and Biochemistry, 44,   2006 11 01 , Pathogenesis-related (PR) proteins are often used as a marker of plant defense reactions. Some endo-1,3-β-glucanase (Gns) genes encode proteins that belong to the PR protein family 2 (PR-2). Although the number of homologous family member genes is significantly greater in hexaploid wheat (Triticum aestivum L.) compared to other model plants, earlier studies did not evaluate the possible contribution of their homologs to hybridization signals in Northern blot analysis. In this study, we have examined whether routine transcriptional analyses of a PR gene is of high reliability or not by isolating six highly similar Gns genes (TaGlb2a, TaGlb2b, TaGlb2c, TaGlb2d, TaGlb2e, and TaGlb2f) and characterizing their expression patterns in detail. While TaGlb2b was shown to be a PR-2 gene, transcription of TaGlb2c and TaGlb2d was not induced upon infection with either powdery mildew (Erysiphe graminis) or head blight (Fusarium graminearum) pathogens; their transcripts were most abundant in healthy spikes (lemmas and in particular paleae). Therefore, in some cases, the conventional analyses do not necessarily provide accurate information on expression pattern of a PR gene in hexaploid wheat. This is also the first report of wheat genes that are specifically expressed in lemma/palea tissues of flowering spikelets. © 2006 Elsevier Masson SAS. All rights reserved.
  • Identification of interspecific hybrids between 'Ichoimo' Chinese yam (D. batatas Decne.) × 'Jinen-jo' Japanese yam (D. japonica Thunb.) by Flow cytometry, TANAKA H., KUSHIKAWA S., FUJIMURA M., KIMURA Y., 75,   2006 09 23
  • B307 Genetically modified Fusarium as a tool to evaluate effects of various agricultural chemicals on initiation of trichothecene biosynthesis, Ochiai Noriyuki, Tokai Takeshi, Sato Masayuki, Fujimura Makoto, Kimura Makoto, 31,   2006 03 10
  • Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea, Michiyo Oshima, Shinpei Banno, Kiyotsugu Okada, Taeko Takeuchi, Makoto Kimura, Akihiko Ichiishi, Isamu Yamaguchi, Makoto Fujimura, Journal of General Plant Pathology, 72,   2006 02 01 , Previously, we cloned a putative osmosensing histidine kinase gene (BcOS1) and revealed that a single amino acid substitution, isoleucine to serine at codon 365, conferred dicarboximide resistance in field isolates of Botrytis cinerea. This point mutation (type I) occurred within the restriction enzyme TaqI site of the wild-type BcOS1 gene. Thus, a procedure was developed for detecting the type I mutation of the BcOS1 gene using a polymerase chain reaction (PCR) in combination with restriction fragment-length polymorphism (RFLP). Diagnosis by PCR-RFLP was conducted on the 105 isolates isolated from 26 fields in Japan. All dicarboximide-sensitive isolates (49 isolates) had the wild-type BcOS1 gene, and the 43 isolates with the type I mutation were resistant to dicarboximides without exception. These data indicate that dicarboximide-resistant isolates with type I mutation are widespread throughout Japan. However, other types of dicarboximide resistance were detected among isolates from Osaka; among the 24 resistant isolates from Osaka, 12 had the BcOS1 gene without the type I mutation. BcOS1 gene sequencing of these resistant isolates classified them into two groups, type II and type III. The type II isolates have three amino acid substitutions within BcOS1p (368Val to Phe, 369Gln to His, and 447Thr to Ser). The type III isolates have two amino acid substitutions within BcOS1p (369Gln to Pro and 373Asn to Ser). These amino acid changes are located on the amino acid repeat domain in BcOS1p. The three types of resistant isolates were all moderately resistant to dicarboximides without significant osmotic sensitivity, and their pathogenicity on cucumber leaves was also very similar to that of the wild-type isolate. © The Phytopathological Society of Japan and Springer-Verlag 2006.
  • Pyrrolnitrin Interferes with Osmotic Signal Transduction in Neurospora crassa, OKADA Akiyoshi, BANNO Shinpei, ICHIISHI Akihiko, KIMURA Makoto, YAMAGUCHI Isamu, FUJIMURA Makoto, Journal of pesticide science, 30, (4) 378 - 383,   2005 11 20 , Pyrrolnitrin, produced by several bacteria that are used in biological control, has an inhibitory effect on the electron transport system of respiration in Neurospora crassa. We have previously described that fludioxonil, a derivative of phenylpyrroles, affects osmotic signal transduction. Both pyrrolnitrin and fludioxonil were highly active against Botrytis cinerea, Fusarium oxysporum, Rhizoctonia solani, and N. crassa. However, a high concentration of pyrrolnitrin (more than 10μg/ml) inhibited the growth of fludioxonil-insensitive fungi such as Pythium ultimum, Phytophthora capsici, and S...
  • (234) Detection of Mutations Conferring Benzimidazole-resistance and Dicarboximide-resistance by Fluorescent Hybridization Probes in Botrytis cinerea(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka), Fujimura M., Hirayama M., Sato M., Noguchi R., Banno S., Kimura M., Yamaguchi I., Annals of the Phytopathological Society of Japan, 71, (3) 247 - 248,   2005 08 25
  • Recent topics on action mechanisms of fungicides, Isamu Yamaguchi, Makoto Fujimura, Journal of Pesticide Science, 30,   2005 08 05 , Plant diseases are damaging to crop production in a temperate and humid climate like that of Japan, which has resulted in the development of many excellent fungicides, but wide-spread use of site-specific fungicides can cause pathogens to develop resistance in the field. While all modern fungicides have been developed through extensive safety evaluation, there is deep public concern about their side effects on non-target organisms. Thus there is a growing interest in non-fungicidal disease-controlling agents since they are supposedly specific to target organisms and less likely to cause resistance. Two groups of non-fungicidal rice blast chemicals are currently on the market; melanin biosynthesis inhibitors (MBIs) and plant defense activators or priming effectors which induce host resistance against the pathogen's attack. Recently, field isolates resistant to MBI-D have emerged and molecular mechanisms of action/resistance are attracting interest. Complex III in the electron transport system of fungal mitochondria which contains a cytochrome b with two ubiquinone binding sites, Qo and Qi, is another novel target of fungicides. Methoxyacrylate fungicides are known to block electron flow by binding to the Qo site. Resistance to Qo inhibitors has been observed in field isolates with a point mutation in the cytochrome b gene (G143A). While dicarboximide fungicides have been applied to protect various crops and vegetables against gray mold and other diseases, their mechanisms of action have remained unclear for many years; recent study revealed that dicarboximides interfere with the osmotic signal transduction pathway consisting of histidine kinase and MAP kinase cascades. The mutations conferring dicarboximide resistance in the field have been identified within the histidine kinase genes in Botrytis cinerea and Alternaria alternata. Thus management strategies for fungicide resistance may become a part of registration requirements worldwide. © Pesticide Science Society of Japan.
  • Recent Topics on Action Mechanisms of Fungicides, YAMAGUCHI Isamu, FUJIMURA Makoto, Journal of pesticide science, 30, (2) 67 - 74,   2005 05 20 , Plant diseases are damaging to crop production in a temperate and humid climate like that of Japan, which has resulted in the development of many excellent fungicides, but wide-spread use of site-specific fungicides can cause pathogens to develop resistance in the field. While all modern fungicides have been developed through extensive safety evaluation, there is deep public concern about their side effects on non-target organisms. Thus there is a growing interest in non-fungicidal disease-controlling agents since they are supposedly specific to target organisms and less likely to cause res...
  • A101 Identification of the genes induced by fludioxonil and other stress in Neurospora crassa, Noguchi Rieko, Banno Shinpei, Kimura Makoto, Yamaguchi Isamu, Fujimura Makoto, 30,   2005 03 10
  • A102 Cross-talk between histidine kinase and cAMP signal transduction in Neurospora crassa, Shiozawa Azusa, Hashimoto Chiho, Ochiai Noriyuki, Banno Shinpei, Kimura Makoto, Yamaguchi Isamu, Fujimura Makoto, 30,   2005 03 10
  • (235) Sensitivity to Fenhexamid and Mutations of Bcerg27 and Bcerg25 Genes in Botrytis cinerea(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004), Noguchi R., Ohba N., Kubo R., Banno S., Sawada H., Kimura M., Yamaguchi I., Fujimura M., Annals of the Phytopathological Society of Japan, 70,   2004 08 25
  • C101 Real-time PCR analysis of the gene expression induced by dicarboximide in Neurospora crassa, Banno Shinpei, Noguchi Rieko, Ochiai Noriyuki, Kimura Makoto, Yamaguchi Isamu, Fujimura Makoto, 29,   2004 03 19
  • C102 Isolation and characterization of seven histidine kinase gene disruptants in N. crassa, Okada Akiyoshi, Ochiai Noriyuki, Banno Shinpei, Kimura Makoto, Yamaguchi Isamu, Fujimura Makoto, 29,   2004 03 19
  • Histidine Kinase Signal Transduction and Fungicide-Resistance in Fungi, Makoto Fujimura, Journal of Pesticide Sciences, 28,   2003 12 01
  • Mutation for Dicarboximide Field Resistance of Botrytis Cinerea in Japan(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo), Oshima M., Fujimura M., Okada K., Takeuchi T., Yamaguchi I., Annals of the Phytopathological Society of Japan, 69,   2003 08 25
  • Histidine Kinases and Fungicide Resistance in Fungi, Fujimura Makoto, 28,   2003 03 14
  • Pyrrolnitrin interferes osmotic signal transduction in N. crassa and plant pathogenic fungi, Okada Akiyoshi, Fujimura Makoto, Ochiai Noriyuki, Usami Ron, Yoshida Yasuhiko, Yamaguchi Isamu, 28,   2003 03 14
  • Mechanism of resistance to dicarboximides and phenylpyrroles, Makoto Fujimura, Noriyuki Ochiai, Takayuki Motoyama, Isamu Yamaguchi, ACS Symposium Series, 808,   2002 12 01 , The osmotic-sensitive os-1, os-2, os4, and os-5 mutants of Neurospora crassa were found resistant not only to iprodione but also to fludioxonil. Stimulation of glycerol biosynthesis by these fungicides observed in the wild-type strain was not induced in the os mutant strains. The os-1 mutant alleles were classified into two groups based on fungicide sensitivity and osmotic stress: Type I (highly resistant to fungicides but moderately sensitive to osmotic stress) and Type II (highly sensitive to osmotic stress but moderately resistant to fludioxonil). Sequence analysis of the os-1 gene in Type I mutant NM233t revealed that the codon for amino acid position 308 was changed to a stop codon in the os-1 gene, suggesting that an os-1 product, which is predicted to be the osmosensor, histidine kinase, may be required for these fungicides to express their antifungal activity. Based on the characterizations of the os mutants in N. crassa, possible mechanisms of laboratory and field dicarboximide-resistance in Botrytis cinerea are also discussed.
  • (81)Functional Analysis of a Histidine Kinase Gene of the Rice Blast Fungus, Motoyama T., Kadokura K., Fujimura M., Yamaguchi I., Annals of the Phytopathological Society of Japan, 68,   2002 08 25
  • (395)Diagnosis of Dicarboximide-Resistance Based on PCR-RFLP in Botrytis cinerea, Oshima M., Fujimura M., Okada K., Takeuchi T., Yamaguchi I., Annals of the Phytopathological Society of Japan, 68, (2) 262 - 263,   2002 08 25
  • Dicarboximide resistant os-4 gene encodes MAPKKK in Neurospora crassa, Ochiai Noriyuki, Fujimura Makoto, Usami Ron, Horikoshi Koki, Yamaguchi Isamu, 27,   2002 02 22
  • Action of a sterol biosynthesis inhibitor, fenhexamid, against the plant pathogenic fungus Botrytis cinerea, Konakawa Chiemi, Mouri Daisaku, Iijima Kazuhide, Fujimura Makoto, Sakurai Akira, 27,   2002 02 22
  • Biotechnology for Plant Protection : Application in Pesticide Research, 26,   2001 08 20
  • Co-existing proteins interfere with the action of nordihydroguaiaretic acid on retrograde Golgi-to-ER protein trafficking in NRK cells and α-glucosidase reaction in vitro, Kazuhito Sato, Kazuhito Sato, Makoto Muroi, Machiko Nakamura, Makoto Fujimura, Akira Takatsuki, Bioscience, Biotechnology and Biochemistry, 65,   2001 04 01 , Induction of retrograde trafficking of mannosidase II and TGN38 in NRK cells and inhibition of α-glucosidase in vitro by nordihydroguaiaretic acid (NDGA) were strongly interfered with by serum, serum albumin, or other unrelated proteins added to the medium or incubation mixture. These observations indicate that NDGA interacts with diverse kinds of proteins, and therefore, pharmacological effects of NDGA at cellular levels should be carefully interpreted.
  • Identification of mutation of two-component histidine kinase gene conferring field-resistance to dicarboximides in Botrytis cinerea, Oshima Michiyo, Fujimura Makoto, Motoyama Takayuki, Yamaguchi Isamu, 26,   2001 02 22
  • Effect of tricyclazole on melanin biosynthesis and cloning of polyketide synthase gene in Neurospora crassa, Banno Shinpei, Oshima Michiyo, Tokai Takeshi, Fujimura Makoto, Yamaguchi Isamu, 26,   2001 02 22
  • Sensitivity to dicarboximides and phenylpyrroles in Candida albicans, Ochiai Noriyuki, Fujimura Makoto, Motoyama Takayuki, Usami Ron, Horikoshi Koki, Yamaguchi Isamu, 26,   2001 02 22
  • Sensitivity to Phenylpyrrole Fungicides and Abnormal Glycerol Accumulation in Os and Cut Mutant Strains of Neurospora crassa, FUJIMURA Makoto, OCHIAI Noriyuki, ICHIISHI Akihiko, USAMI Ron, HORIKOSHI Koki, YAMAGUCHI Isamu, Journal of pesticide science, 25, (1) 31 - 36,   2000 02 20 , Neurospora osmotic sensitive strains with os-1, os-2, os-4 and os-5 mutations showed cross-resistance to dicarboximides, aromatic hydrocarbons and fludioxonil. The os-2, os-4, and os-5 mutant strains were highly resistant to them, while the os-1 mutant strain was moderately resistant and its growth was inhibited by fludioxonil (LD_<50> : 0.087μg/ml) and iprodione (LD_<50> : 14μg/ml). Another osmotic sensitive mutation cut did not give resistance to these fungicides. The conidia of wild-type and cut strains swelled and burst without germination on the medium containing fludioxonil, and burst...
  • Effect of dicarboximides and phenylpyrroles on glycerol accumulation in Neurospora osmotic sensitive mutants, Fujimura Makoto, Yamaguchi Isamu, 24,   1999 03 01
  • Topic 4 Mode of Action and Resistance, Journal of pesticide science, 23, (4) 446 - 454,   1998 11 20
  • Action Mechanism of Diethofencarb to a Benzimidazole-Resistant Mutant in Neurospora crassa, FUJIMURA Makoto, KAMAKURA Takashi, YAMAGUCHI Isamu, Journal of pesticide science, 17, (4) 237 - 242,   1992 11 20 , In the presence of 0.5μg/ml diethofencarb, conidia of a benzimidazole-resistant mutant of Neurospora crassa germinated, with distorted, swollen germ tubes. Diethofencarb induced scattered nucleus and inhibited mitotic nuclear division in the resistant strain. The morphological abnormality was quite similar to the one observed in the wild-type strain treated with carbendazim (MBC). Diethofencarb, however, did not morphologically affect the germ tubes and nuclei of a wild-type strain. Diethofencarb formed a complex in vitro with a protein present in the mycelial extracts of the resistant muta...
  • Action Mechanism of Diethofencarb to a Benzimidazole-Resistant Mutant in Neurospora crassa, Makoto Fujimura, Takashi Kamakura, Isamu Yamaguchi, Journal of Pesticide Science, 17,   1992 01 01 , In the presence of 0.5 g/ml diethofencarb, conidia of a benzimidazole-resistant mutant of Neurospora crassa germinated, with distorted, swollen germ tubes. Diethofencarb induced scattered nucleus and inhibited mitotic nuclear division in the resistant strain. The morphological abnormality was quite similar to the one observed in the wild-type strain treated with carbendazim (MBC). Diethofencarb, however, did not morphologically affect the germ tubes and nuclei of a wild-type strain. Diethofencarb formed a complex in vitro with a protein present in the mycelial extracts of the resistant mutant. The binding protein of the resistant strain was retained on a DEAE-Sephadex A-50 and eluted with 0.5 m kc1. The molecular weight of the binding protein was estimated 105,000 by gel filtration on Sephacryl S-200 chromatography. The data well coincide with those on the MBC-binding protein in the wild-type strain, suggesting that diethofencarb was selectively toxic to the benzimidazole-resistant strain by binding to the tubulin. © 1992, Pesticide Science Society of Japan. All rights reserved.
  • Sensitivity of Neurospora crassa to benzimidazoles and N-phenylcarbamates: Effect of amino acid substitutions at position 198 in β-tubulin, Makoto Fujimura, Takashi Kamakura, Hirokazu Inoue, Satoru Inoue, Isamu Yamaguchi, Pesticide Biochemistry and Physiology, 44,   1992 01 01 , Diethofencarb-resistant revertants have been isolated from the Neurospora crassa mutant F914 which shows both carbendazim (MBC) resistance and diethofencarb sensitivity. Revertant strains FR422 and FR424 were true revertants showing high resistance to N-phenylcarbamates and sensitivity to benzimidazoles, the same as wild-type. However, other revertant strains, FR511, FR513, FR521, FR421, and FRB3, were more resistant to diethofencarb than the strain F914 but less resistant to diethofencarb than the wild-type strain. These moderately resistant strains were also more resistant to benzimidazoles than the parent strain F914, Strains FR421 and FR521 were also resistant to griseofulvin. All the diethofencarb-resistant mutations were linked to the Bml locus that encodes β-tubulin. The β-tubulin gene of FRB3 strain was cloned and sequenced, and an amino acid substitution at the position 198 was found. In the strains FRB3, F914, and wild-type, the amino acids at position 198 were Lys, Gly, and Glu, respectively. A new β-tubulin gene with Ala at position 198 was constructed by site-directed mutagenesis. This gene transformed the wild-type strain to MBC resistance, while transformation of the F914 strain with this gene resulted in growth on the medium containing 0.5 μg/ml diethofencarb but not on that containing 75 μg/ml diethofencarb. The F914 strain transformed by β-tubulin genes of the wild-type strain and the FRB3 strain grew even on medium containing 75 μg/ml diethofencarb. These data suggest that the presence of Glu at position 198 in β-tubulin is responsible for MBC sensitivity. Different amino acid substitutions at position 198 confer different sensitivity to diethofencarb and the order of amino acids which result in increasing sensitivity are Glu, Lys, Ala, and Gly (most sensitive). These approaches should work in clarifying the function of β-tubulin and also aid in the design of new potent fungicides. © 1992.

Books etc

  • Fungicide Resistance in Plant Pathogens: Principles and a Guide to Practical Management
    Springer  2015 09 01
  • Neurospora: Genomics and Molecular Biology
    Caister Academic Press  2013 01 01

Conference Activities & Talks

  • Null Mutations of OS-1 Like Histidine Kinase Gene Confer Resistance to Dicarboximides and Phenylpyrroles in Corynespora cassiicola, Yamaguchi Y., Tsukada Y., Horiuchi A., Fujimura M., Asian Conference on Plant Pathology 2017,   2017 09 14
  • Construction and application of pyrosequencing assays to detect fungicide-resistance mutations in cucumber pathogens, Aimi Horiuchi, Tsuyoshi Shinpuku, Yoshihito Tsukada, Yusuke Yamaguchi, Ryouko Satake, Makoto Fujimura, Asian Conference on Plant Pathology 2017,   2017 09 14
  • Mitochondrial genome sequences of the hybrid Verticillium longisporum A1/D1 and A1/D3 strains, Ryouko Satake, Ryohei Shoda, Mina Yamaguchi, Yusuke Yamaguchi, Makoto Fujimura,   2017 09 14
  • Isolation and characterization of QoI-resistant mutants with the cytbG143S and cytbG137E in Neurospora crassa, Makoto Fujimura,   2016 03 18
  • Monitoring of fungicide-resistant populations in cucumber corynespora leaf spot fungus using pyrosequencing SNP assays, Tsuyoshi Shinpuku, Shinpei Banno, Shiho Kasuya, Makoto Fujimura,   2014 03 15
  • Transcriptional regulation of the erg genes response to ergosterol biosynthesis inhibitors, azole and morpholine,, Moto Miyashita, Mizuki Takenaka, and Makoto Fujimura., Neurospora 2014,   2014 03 09
  • MSN-1 is a transcription factor regulated by MAK-1 MAP kinase in Neurospora crassa., Masayuki Kamei, Masakazu Takahashi, Akihiko Ichiishi, and Makoto Fujimura, Neurospora 2014,   2014 03 08

Research Grants & Projects

  • Functional analysis on fungal histidine kinases based on genome information and its application on fungicide screening, Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Makoto FUJIMURA, Neurospora crassa, a filamentous fungus, has eleven histidine kinase genes in its genome, while Saccharomyces cerevisiae has only one histidine kinase gene. Most of the histidine kinases were highly conserved in filamentous fungi including Magnaporthe girisea, suggesting that fungi use many kinds of histidine kinases for environmental response and differentiation. We carried out gene disruption in N. crassa by RIP mutation, and isolated and characterized eight kinds of histidine kinase gene disruptants. However, all disruptants, except os-1 gene, did not show abnormality in mycelial growth, osmotic or oxidative stress, fungicide sensitivity, and fertility. These results suggested the possibility of redundancy of fungal histidine kinase. To screen the isolates with several histidine kinase gene disruption by genetic cross, we constructed the PCR method to detect RIP mutations for each histidine kinase gene.Os-1 histidine kinase is responsible for osmotic stress response and dicarboximide-resistance. We identified os-4 (MAPKK kinase) and os-5 (MAPK kinase) genes, which are regulated down stream of os-1 histidine kinase. Furthermore, we revealed that transcription of NcCTT1 and NcGPD1 genes was activated by dicarb6ximide fungicides and their activation was dependent on os-1 pathway. Based on above results, construction of the screening system for histidine kinase inhibitors is undergoing.
  • Melanin biosynthesis and its biological function as heat persistence in plantpathogens and other fungi, Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Makoto FUJIMURA, Melanin biosynthesis inhibitors, such as carpropamid and diclocymet, affected conidial formation in Pyricularia oryzae. In addition, the conidia on the medium containing fungicides increased the sensitivity to heat treatment. Reduced heat persistence induced by fungicides was not observed in fungicide resistant isolates, suggesting correlation between melanization and heat persistence. In Neurospora crassa, black pigment develops in sexual organs such as perithecia and ascospore, and ascospore showed high resistance to heat treatment. On the other hand, ascospore of the per-1 mutant, melanin deficient strain, was sensitive to heat treatment. The wild-type strain treated by tricyclazole accumulated 2-HJ, a shunt product of DHN-melanm. The DHN-melanin synthesis genes, such as PKS, SDH, and HNR, were clones and characterized. The per-1 mutant strain transformed with the wild-type PKSgene recovered the ability of melanin biosynthesis and heat resistance. The mutation within the PKSgene of the per-1 mutant strain was identified as ^<210>arg to stop codon. The transcription of melanin biosynthesis genes was induced by fertilization not by nutrient starvation in sexual cycle of N.crassa.
  • DICARBOXIMIDE RESISTANCE AND OSMOTIC REGULATION IN PLANT PATHOGENS, Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Makoto FUJIMURA, Dicarboximides have been applied on various crops against Botrytis cinerea. However, dicarboximide-resistant isolates of B. cinerea appeared on a variety of crops worldwide. To study mode of action and resistant mechanism of dicarboximides we use Neurospora crassa as a model fungus. The osmotic sensitive os-1, os-2, os-4 and os-5 mutants of Neurospora crassa were found to be resistant not only to iprodione but also to fludioxonil. Stimulation of glycerol biosynthesis by these fungicides observed in the wild-type strain was not induced in os mutant strains. The os-1 mutant alleles were classified into two groups based on sensitivity to fungicides and osmotic stress: Type I (highly resistant to fungicides but moderately sensitive to osmotic stress) and Type II (highly sensitive to osmotic stress but moderately resistant to fludioxonil). Sequence analysis of os-1 gene indicate that type I strains were null mutants. Thus we concluded that a os-1 product is essential for these fungicides to express their antifungal activity. Type II mutants have single amino acid substitutions within amino-acid repeats of os-1 gene, suggesting the amino-acid repeats may play a important rule for osmoreguylation.